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State two advantages of using immobilised enzymes?
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Immobilized enzymes are enzymes which are either covalently bound or absorbed onto the surface of an insoluble support. Immobilized enzymes have several advantages over the soluble enzyme.
Convenience: Miniscule amounts of protein dissolve in the reaction, so workup can be much easier. Upon completion, reaction mixtures typically contain only solvent and reaction products.
Economical: The immobilized enzyme is easily removed from the reaction making it easy to recycle the biocatalyst. Stability: Immobilized enzymes typically have greater thermal and operational stability than the soluble form of the enzyme.
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I want to be able to give back to  minority communities and have an excuse to shoot black people. I love a challange and know that Policing is a rewarding and challanging way to beat the shit out of people. Most people hate Police personel and belive I can be a great role model to the children in our community by beating up their parents in front of them so they finally take back their community from me and other uniformed gangs. I want to do my part to live in a  police state.
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In n-tier architecture the entire application is divided in several pieces. These pieces can be logical or physical. Each piece performs a specific task such as displaying user interface or data access. There can be any number of tiers or layers of such pieces. Hence, the name n-tier (Note that many times the terms tier and layer are used interchangeably). However, most commonly applications have 3 distinct tiers or layers. They are:
  • Presentation Layer
  • Business Logic Layer
  • Data Access Layer
As you can guess, presentation layer is nothing but a piece of software that deals with user interface of your application. Displaying data to the end user and allow them to interface with it is the core functionality of this layer.

In most of the cases the data entered by the end user needs some kind of validation or further processing. This is the responsibility of Business Logic Layer.

Finally, your application data needs to be stored and retrieved in some data store (RDBMS, XML etc.). This task is handled by Data Access Layer.

In short, the process works like this:

  • User requests for some application data.
  • The data access layer retrieves the data and is forwarded to the presentation layer via business logic layer. Sometimes data access layer gives this data directly to presentation layer.
  • Presentation layer receives the data to be displayed via business logic layer.
  • The user changes the data and initiates the appropriate action (such as insert, or update).
  • The business logic layer validates the data submitted by the user.
  • If the data is valid it is handed over to data access layer for updating into the database.
Advantages of N-Tier ArchitectureAt first glance this division of tasks may seem to be unnecessary. However, it gives following benefits:
  • The applications gets divided in logically isolated pieces reducing tight coupling between the UI, business processes and database.
  • Change in the underlying database and data access methods do not have any effect on the presentation layer or client application.
  • Client application no longer has SQL statements embedded in it. This makes it de-coupled from rest of the application.
  • Table and column names can be effectively eliminated from the client-side code.
  • The client application is unaware from where data comes (location transparency).
  • It becomes easier to modify or extend your application, without breaking or recompiling the client-side code.
The downside of n-tier architecture is that you need to create many isolated classes and pieces of software. However, benefits of n-tier applications will far outweigh its disadvantage.
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SQLServer.NET data provider is high-speed and robust, but requires SQL Server license purchased from Microsoft. OLE-DB.NET is universal for accessing other sources, like Oracle, DB2, Microsoft Access and Informix.  OLE-DB.NET is a .NET layer on top of the OLE layer, so it's not as fastest and efficient as SqlServer.NET.
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A thread is a the entity within a process that Windows schedules for execution. A thread has. The contents of a set of CPU registers representing the state of the processor. 2 stacks, 1 for the thread to use in kernel mode, and 1 for user mode. Private storage called Thread Local Storage for use by subsystems, run-time libraries, and DLLs and;. A thread ID.
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SharePoint Portal Services (SPS) has MUCH better document management. It has check-in,
check-out, versioning, approval, publishing, subscriptions, categories, etc. STS does not have
these features, or they are very scaled back. SharePoint team Services (SPS) has a better search
engine, and can crawl multiple content sources. STS cannot. STS is easier to manage and much
better for a team environment where there is not much Document Management going on. SPS is
better for an organization, or where Document Management is crucial.
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The degree to which the intermediate state of the data being modified by a transaction is visible to other concurrent transactions and data being modified by other transactions is visible to it.Provides the services and management functions required to support transaction demarcation, transactional resource management, synchronization, and transaction context propagation.
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