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It refers to the grid of nucleic acid molecules of known sequence linked to a solid substrate, which can be probed with a sample containing either messenger RNA or complementary DNA from a cell or tissue to reveal changes in gene expression relative to a control sample.
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Sunday, April 27, 2008
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GMO - Genetically modified organism (GMO) is Genetic material of an organism is  altered with the help of genetic engineering techniques generally known as recombinant DNA technology. DNA molecules from one source is combined with the DNA of other in vitro into one molecule to create a new gene. The recombinant DNA is then transferred into an organism causing the expression of modified or novel traits. The product is also known as an Genetically Engineered Organism or GEO.

Examples Transgenic animals (mice, fish),  transgenic plants (BT Cotton, Flavour Savour Tomato), or various microbes (fungi and bacteria) . The production and use of GMOs are ivolved in biology or medicine, for the production of pharmaceuticals and industrial enzymes, and for direct, and often controversial, applications aimed at improving human health (e.g., gene therapy) or agriculture (e.g., golden rice). For example, a gene from a jellyfish, encoding a fluorescent protein called GFP, can be physically linked and thus co-expressed with mammalian genes to identify the location of the protein encoded by the GFP-tagged gene in the mammalian cell. The indispensable use of GMO is in many areas of research, such as to study the mechanisms of human and other diseases .
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Thursday, March 20, 2008
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Recombinant DNA is DNA that has been created artificially.
DNA from two or more sources is incorporated into a single recombinant molecule.
BamHI cuts the same site on both molecules
5' GGATCC 3'
3' CCTAGG 5'.
The ends of the cut have an overhanging piece of single-stranded DNA.
These are called "sticky ends" because they are able to base pair with any DNA molecule containing the
complementary sticky end.
In this  both DNA preparations have complementary sticky ends and thus can pair with each other when mixed.
DNA ligase covalently links the two into a molecule of recombinant DNA.
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Thursday, March 20, 2008
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Radiation, as used in physics, is energy in the form of waves or moving subatomic particles. Radiation can be classified as ionizing or non-ionizing radiation, depending on its effect on atomic matter. The most common use of the word "radiation" refers to ionizing radiation. Ionizing radiation has enough energy to ionize atoms or molecules while non-ionizing radiation does not. Radioactive material is a physical material that emits ionizing radiation.
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Monday, January 21, 2008
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When blood vessels are cut or damaged, the loss of blood from the system must be stopped before shock and possible death occur. This is accomplished by solidification of the blood, a process called coagulation or clotting.

A blood clot consists of
  • a plug of platelets enmeshed in a
  • network of insoluble fibrin molecules.
Platelet aggregation and fibrin formation both require the proteolytic enzyme thrombin. Clotting also requires:
  • calcium ions (Ca2+)(which is why blood banks use a chelating agent to bind the calcium in donated blood so the blood will not clot in the bag).
  • about a dozen other protein clotting factors. Most of these circulate in the blood as inactive precursors. They are activated by proteolytic cleavage becoming, in turn, active proteases for other factors in the system.
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Monday, January 21, 2008
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Osmosis is the movement of water molecules from an area of  high concentration to an area of low
concentration.

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Sunday, January 20, 2008
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Dick
Thursday, January 31, 2008 10:11 AM
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In recent years astronomers and biologists have discovered that many of the molecules essential for life - purines, amino acids, etc. - seem to be widely distributed in the universe, and hence might gain a foothold on other suitable planets. Some maintain that these seeds of life, "transpermia," wafting through the universe are the most likely reason for the origin of life on earth. But whether life on this planet began by a unique, fortuitous combination of elements or was jump-started by transpermia from afar, bear in mind that there is no evidence of any form of life during earth's first billion years. Only at about 3.8 billion years ago have we found evidence for the first life forms, prokaryotes - simple cells without an organized nucleus.    
        From one point of view we are minor actors in a scheme of which we are largely unaware. From another point of view bacteria illustrate how very simple organisms can become spectacularly successful - aided immensely, of course, by invading complex organisms, and bending these hosts to their own purposes.                               

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Tuesday, October 16, 2007