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In RFLP analysis, the DNA of an organism is cut up into fragments using restriction enzymes. A large number of short fragments of DNA will be produced.
Restriction enzymes always cut at the same base sequence.
Because no two individuals have identical DNA, no two individuals will have the same length fragments. For
example, the enzyme EcoRI always cuts DNA at the sequence GAATTC.
Different people  have different numbers of this particular sequence and will therefore have different fragment
lengths.  some of them will be at different locations on the chromosome.
DNA were placed on the gelatin.
The DNA bands must be stained to make them visible.
Ethidium bromide-stained DNA will fluoresce when illuminated with UV light.
PCR techniques are used to produce sufficient quantities of DNA for this technique.

Some of the techniques used to transfer DNA  cells into animals and plants are

1) Bacterial carriers
2) Biolistics
3) Calcium phosphate precipitation
4) Electroporation
5) Gene silencing
6) Gene splicing
7) Lipofection
8) Microinjection
9)Viral carriers.

Cloned Animals

For finding DNA fragments: included is agarose, TAE buffer, ethidium bromide.  Then a loading  dye is added to each lane, and also a ladder must be  included for comparison.

DNA profiling technology can be used to identify any genetic (hereditary) disease, due to certain abnormality in the genetic makeup, which might be the cause of the disease.

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